Coding

Part:BBa_K4714002:Design

Designed by: Mufeng Li   Group: iGEM23_BAID-China   (2023-10-12)


CBM3-CBM3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 631
    Illegal PstI site found at 805
    Illegal PstI site found at 1330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 631
    Illegal PstI site found at 805
    Illegal PstI site found at 1330
    Illegal NotI site found at 1301
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 631
    Illegal PstI site found at 805
    Illegal PstI site found at 1330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 631
    Illegal PstI site found at 805
    Illegal PstI site found at 1330
    Illegal NgoMIV site found at 786
    Illegal NgoMIV site found at 978
    Illegal AgeI site found at 91
    Illegal AgeI site found at 301
    Illegal AgeI site found at 2233
    Illegal AgeI site found at 2443
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His-tag is added to N-terminus. The flexible linker GGGGS2x was used between CBM3. The sequences have been codon optimized based on the E. coli expression system.


Source

CBM3 coding sequence from iGEM BBa_K4011000, Oat 11s Globulin from iGEM BBa_K4714003

References