![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4714002:Design
Designed by: Mufeng Li Group: iGEM23_BAID-China (2023-10-12)
CBM3-CBM3
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 631
Illegal PstI site found at 805
Illegal PstI site found at 1330 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 631
Illegal PstI site found at 805
Illegal PstI site found at 1330
Illegal NotI site found at 1301 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 631
Illegal PstI site found at 805
Illegal PstI site found at 1330 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 631
Illegal PstI site found at 805
Illegal PstI site found at 1330
Illegal NgoMIV site found at 786
Illegal NgoMIV site found at 978
Illegal AgeI site found at 91
Illegal AgeI site found at 301
Illegal AgeI site found at 2233
Illegal AgeI site found at 2443 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
His-tag is added to N-terminus. The flexible linker GGGGS2x was used between CBM3. The sequences have been codon optimized based on the E. coli expression system.
Source
CBM3 coding sequence from iGEM BBa_K4011000, Oat 11s Globulin from iGEM BBa_K4714003